Please help me to develop protocol for DNA extration from Potato chips.
Thanks a lot.
0
6 replies to this topic
#2
phage434
-
- Global Moderators
-
- 1,805 posts
Veteran
130
Excellent
Excellent
Posted 29 June 2012 - 07:51 AM
I'd start with a chloroform wash to remove as much of the lipid as possible. Perhaps you could do that twice, along with crushing the chips.
I'd then follow a standard CTAB extraction.
I'd then follow a standard CTAB extraction.
#3
Potato
-
- Members
-
- 3 posts
member
0
Neutral
Neutral
Posted 29 June 2012 - 07:51 AM
Thanks a lot. That's what I am thinking to start.
Best wishes.
Best wishes.
#4
hobglobin
-
- Active Members
-
- 5,249 posts
Growing old is mandatory, growing up is optional...
60
Excellent
Excellent
Posted 29 June 2012 - 07:54 AM
did you try out already any protocol? If not I'd just start first with a protocol established in your lab...
My first idea would be to remove as much as oil as possible before e.g. by crushing them to smaller pieces and then putting it on absorptive paper (e.g. kitchen towel)....
My first idea would be to remove as much as oil as possible before e.g. by crushing them to smaller pieces and then putting it on absorptive paper (e.g. kitchen towel)....
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#5
Potato
-
- Members
-
- 3 posts
member
0
Neutral
Neutral
Posted 29 June 2012 - 07:58 AM
@Hobglobin: Never did it before. Was thinking to try it with our usual CTAB protocol may do some modifiactions as needed & from the help of all of you guys.
Thanks a lot.
Best Wishes.
Thanks a lot.
Best Wishes.
#6
hobglobin
-
- Active Members
-
- 5,249 posts
Growing old is mandatory, growing up is optional...
60
Excellent
Excellent
Posted 29 June 2012 - 08:14 AM
good luck and using chloroform is surely a more thoroughly method....
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#7
bob1
-
- Global Moderators
-
- 4,347 posts
Thelymitra pulchella
224
Excellent
Excellent
Posted 29 June 2012 - 05:43 PM
Are these chips as in fish and chips or chips as in wafers of potato (crisps if you are british)?
If they are the latter, you will probably struggle with DNA extraction as I would expect the DNA to be quite damaged from the heating during cooking.
If they are the latter, you will probably struggle with DNA extraction as I would expect the DNA to be quite damaged from the heating during cooking.
