I feel really frustrated with these strange things in my culture flasks. They are floating in the culture medium. Their sizes are comparable with my cancer cells(PC-3 and A549). They reappear even though I wash the cells many times before re-adding the medium. And it seems that there will be more and more such floating things a few days later. Are my cells contaminated??
0
Unknown floating substances in culture flask
Started by vivianyuwl, Jun 29 2012 06:36 AM
floating object cell culture
1 reply to this topic
#1
vivianyuwl
-
- Members
-
- 1 posts
member
0
Neutral
Neutral
Posted 29 June 2012 - 06:36 AM
hi all,
I feel really frustrated with these strange things in my culture flasks. They are floating in the culture medium. Their sizes are comparable with my cancer cells(PC-3 and A549). They reappear even though I wash the cells many times before re-adding the medium. And it seems that there will be more and more such floating things a few days later. Are my cells contaminated??
I feel really frustrated with these strange things in my culture flasks. They are floating in the culture medium. Their sizes are comparable with my cancer cells(PC-3 and A549). They reappear even though I wash the cells many times before re-adding the medium. And it seems that there will be more and more such floating things a few days later. Are my cells contaminated??
#2
bob1
-
- Global Moderators
-
- 4,345 posts
Thelymitra pulchella
223
Excellent
Excellent
Posted 29 June 2012 - 05:59 PM
These are dead/dying cells. ANy population of cells will have a few.
Also tagged with one or more of these keywords: floating object, cell culture
 |
Protocols and Techniques Forums →
Cell Biology →
Debris in cell cultureStarted by Guest_laramadriz_* , 17 May 2013  cell culture
|
|
|
|
|
|
Protocols and Techniques Forums →
Tissue and Cell Culture →
Cell-repellent coating / surfaces - Growing cells on beadsStarted by Guest_flooff_* , 14 May 2013 coating, MDCK, cell culture and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Tissue and Cell Culture →
C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!!Started by Guest_KayW_* , 12 May 2013 cell culture
|
|
|
|
|
|
Protocols and Techniques Forums →
Tissue and Cell Culture →
Spontaneous immortalization of mouse cells in vitroStarted by Guest_Epigeneticist_* , 05 May 2013 Cell Culture, Mouse, Human and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Stem Cell →
problems in culturing mice mesenchymal stem cellStarted by Guest_mesenchymal stem cell_* , 29 Apr 2013 mesenchymal stem cell and 1 more...
|
|
|
