Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Sub G1 in cell cycle

flow cytometry

  • Please log in to reply
2 replies to this topic

#1 majidatf

majidatf

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 29 June 2012 - 12:06 AM

Hi to all
Could everyone please tell me about the subG1 cells in flow cytometry histogram? I mean what is the identity of these cell? are they apoptotic cells or not?
thanks

#2 Dennis_Simpson

Dennis_Simpson

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 04 July 2012 - 02:58 PM

They might be apoptotic. This measurement is used sometimes as a proxy for apoptosis. Depending on what was done to the culture they could also arise from mitotic collapse or other non-disjunction type errors during mitosis.

#3 Danint

Danint

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 13 July 2012 - 07:25 AM

You could try using Annexin V-FITC as an indicator of potential apoptosis in these cells? Or if you have a proper FACS, could you not collect this sub population and run an apoptosis-related western blot (caspase-3, Bax. Bcl-2 etc)?





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.