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[chiselv8]

Hi, I'm working with DMSO as a vehicle in drug treatment in LNCaP cells, when I use dmso at a concentration greater than 0.5% cells have morphological alterations, I see them rounded and they grow in clusters, but cell viabilidad and proliferation are not compromised, Has anyone seen any similar effect?

Thank you!!

[bob1]

Yes, some cell types are more sensitive to DMSO than others -  think 0.01% is about the lower limit of sensitivity for most cell lines and 0.5% about the maximum you would want to go.

Have you tried other vehicles such as dimethylformamide (DMF)?