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DMSO induces cluster growing in LNCaP cells?

Dmso Cell culture

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#1 chiselv8

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Posted 28 June 2012 - 05:27 PM

Hi, I'm working with DMSO as a vehicle in drug treatment in LNCaP cells, when I use dmso at a concentration greater than 0.5% cells have morphological alterations, I see them rounded and they grow in clusters, but cell viabilidad and proliferation are not compromised, Has anyone seen any similar effect?

Thank you!!

#2 bob1

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Posted 29 June 2012 - 11:21 PM

Yes, some cell types are more sensitive to DMSO than others - think 0.01% is about the lower limit of sensitivity for most cell lines and 0.5% about the maximum you would want to go.

Have you tried other vehicles such as dimethylformamide (DMF)?





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