I'm following the Cold Spring Harbor labs' protocol for PEG purification of DNA. The PEG-MgCl2 solution they describe is as follows (from http://cshprotocols....06/1/pdb.rec382)
40% (w/v) polyethylene glycol (PEG 8000)
30 mM MgCl2
Dissolve 40 g of PEG 8000 in a final volume of 100 ml of 30 mM MgCl2. Sterilize the solution by passing it through a 0.22-μm filter, and store it at room temperature.
However, I only have PEG of MW 3350 in the lab, not PEG of MW 8000. Will it still work with PEG 3350?
Why/why not? I don't know how PEG works to precipitate DNA and I would really appreciate it if someone who knew explained it to me.
Edited by hogthehedge, 28 June 2012 - 03:18 AM.