Problem with visibility on third comb in gelsGel visibility
Posted 27 June 2012 - 02:41 PM
Im using 3 combs with 36 wells each on every gel I run. On several of the gels I run I can clearly see the DNA on first two combs when I take the picture under UV light but the DNA samples on the third and final comb are hardly visible.
Could this be a problem with the buffer or gel? Does anybody know what it could be? Any help or comments would be greatly appreciated.
I attached a picture so you can see what im talking about.
Posted 27 June 2012 - 03:22 PM
Posted 29 June 2012 - 08:34 AM
That's very interesting...since we always stain our gels after running them it was never an issue...
Posted 23 September 2012 - 09:05 AM
Posted 23 September 2012 - 12:26 PM