Problem with visibility on third comb in gelsGel visibility
Posted 27 June 2012 - 02:41 PM
Im using 3 combs with 36 wells each on every gel I run. On several of the gels I run I can clearly see the DNA on first two combs when I take the picture under UV light but the DNA samples on the third and final comb are hardly visible.
Could this be a problem with the buffer or gel? Does anybody know what it could be? Any help or comments would be greatly appreciated.
I attached a picture so you can see what im talking about.
Posted 27 June 2012 - 03:22 PM
- Papaver likes this
Posted 29 June 2012 - 08:34 AM
Because the dye (e.g. EB) moves the opposite way from your DNA, that is why the bands at the bottom are not visible anymore.
That's very interesting...since we always stain our gels after running them it was never an issue...
Posted 23 September 2012 - 09:05 AM
Posted 23 September 2012 - 12:26 PM