hi, i finally manage to express my beta glucosidase gene in e coli rosettagami2 plys s. during lysis, i lysed the pellet, and i gpt some sample from this my crude extract. then i add some protamine sufate and i centrifuge to got the second sample ant use the pellet for the third sample. when i performed western blot, i see my band at 55 kDa although bioinformatic analysis say it is 63kDa. but among the three samples, i saw only a band and it is the one before protamine sulfate . now i wonder about if i inhibit the protein or the band was incorrect? please helpppppp!!!!!!!!!!!!!!!!
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mdfenko
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an elder
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Excellent
Excellent
Posted 28 June 2012 - 08:20 AM
molecular weight determination with sds-page is not always (often?) accurate (for various reasons).
and you may not be taking post translational modifications into account.
and you may not be taking post translational modifications into account.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
