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plant extraction procedure for protein


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3 replies to this topic

#1 shivangipurohit

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Posted 27 June 2012 - 10:38 AM

what is the full extraction procedure for plant parts specially leaf and legume seeds...for sds electrophoresis.

#2 proteaMatt

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Posted 28 June 2012 - 07:45 AM

So you are looking for a protocol to lyse cells from leaves and legumes?

I have attached an application note that we compiled recently regarding cell lysis from peanuts. Perhaps this can be of some use to you. As an aside, you probably won't need to do the hexane lipid extraction unless you are having problems with resolving bands on your gel. Attached File  Application Note_Protea cell lysis kits - 03.23.12.pdf   481.83KB   587 downloads

Edited by proteaMatt, 28 June 2012 - 12:49 PM.

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#3 shivangipurohit

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Posted 28 June 2012 - 10:27 PM

thank u for pdf...i want protocol for extraction procedure for protein from green gram seed and its leaf....TCA extraction is fine or need any other method to b followed?

#4 proteaMatt

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Posted 29 June 2012 - 06:53 AM

I don't have any experience with using TCA for protein extraction from plant cells, so I can't really speak to its efficacy. I'm sure if you google search TCA protein extraction you can turn up a few results.

My experience is primarily confined to using surfactant based lysis methods. The pdf file I linked above shows usage of our acid labile surfactants (which are alternatives to surfactants such as SDS, CHAPS, and Triton-X) to extract total protein from peanut cells. We have also used the same method to extract total protein from various other types of nuts as well as Arabidopsis sp. leaves. I think a surfactant based method would be suitable for your purposes if you choose to go that route.

Edited by proteaMatt, 29 June 2012 - 06:54 AM.

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