plant extraction procedure for protein
Posted 27 June 2012 - 10:38 AM
Posted 28 June 2012 - 07:45 AM
I have attached an application note that we compiled recently regarding cell lysis from peanuts. Perhaps this can be of some use to you. As an aside, you probably won't need to do the hexane lipid extraction unless you are having problems with resolving bands on your gel. Application Note_Protea cell lysis kits - 03.23.12.pdf 481.83KB 1000 downloads
Edited by proteaMatt, 28 June 2012 - 12:49 PM.
Posted 28 June 2012 - 10:27 PM
Posted 29 June 2012 - 06:53 AM
My experience is primarily confined to using surfactant based lysis methods. The pdf file I linked above shows usage of our acid labile surfactants (which are alternatives to surfactants such as SDS, CHAPS, and Triton-X) to extract total protein from peanut cells. We have also used the same method to extract total protein from various other types of nuts as well as Arabidopsis sp. leaves. I think a surfactant based method would be suitable for your purposes if you choose to go that route.
Edited by proteaMatt, 29 June 2012 - 06:54 AM.