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Secondary Ab is M.I.A. ... how long can I leave my membrane in wash buffer?

western blotting immunoblotting antibodies electrophoresis secondary antibody

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#1 cpadding

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Posted 27 June 2012 - 09:37 AM

Hi guys,

So one of my lab mates used the last of the secondary I planned to use today without replacing it. I started rinsing my membranes before checking to make sure I had the antibody I needed, rookie mistake. Is it okay to leave the membranes in my wash buffer (same as the antibody buffer I use) at 4 degC for an extra day or so? Any advice would be appreciated!

Thanks

#2 bob1

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Posted 27 June 2012 - 12:57 PM

The membranes will be fine in the wash buffer, but any antibody you have on there will get progressively washed off so you may have to re-do the primary. You could leave in PBS or TBS which would be better than a buffer with detergent.





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