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Metabolic quenching


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#1 PARUL MEHROTRA

PARUL MEHROTRA

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Posted 27 June 2012 - 02:47 AM

Hi,

I have to do an assay where I need quench metabolic activity of cells infected with a parasite, lyse the cells and isolate the parasite from the host cells to study a particular parasite metabolite.

Generally I quench metabolic activity using chilled methenol : water (80:20). However the solvent is not sufficient to lyse the host cells instantaneously. what detergent (Or any other compound) should i add to the chilled solvent to lyse the host cell and quench the metabolic activity simultaneously.?????

I have tried DMSO, Triton X100 and Tween. Nothing works at the temperature i want to maintain. :(

Thanks in advance !!


Parul




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