Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

Unknown contaminant in S. cerevisiae culture

S. cerevisiae contamination cocci grampositive motile

  • Please log in to reply
1 reply to this topic

#1 Jardy



  • Members
  • Pip
  • 1 posts

Posted 27 June 2012 - 02:03 AM

The problem I´m dealing with is a contamination in my S. cerevisiae cultures (GG 595, W303, BY4741) of unknown nature which suddenly occurred in our lab some months ago. It looks like G+ motile cocci swimming in the culture. All of our attempts to isolate them (by straining or in semi-liquid media) have been unsuccessful. They´re also resistant to ampicilin, kanamycin and chloramphenicol. Yeast cells in the presence of contaminants shrivel up and don´t grow well (especially, when used for transformation - LiAc method).

Do you have any suggestions? Which species this could be and how to identify them?
Thanks a lot.

#2 gebirgsziege


    I like fungi

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 392 posts

Posted 27 June 2012 - 03:53 AM

you should be able to get rid off them by streaking the culture on media without antibiotics. I would suggest at least 4 times subculturing. Make sure that your culture media are not contaminated and that you pick one single colony as soon as you see them, grow them, and check for purity.

Otherwise get a new batch of yests to grow - usually your lab should have a uncontaminated stock somewhere in the freezer.
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.