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Exact molecular weight?

electrophoresis

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#1 @bhijit

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Posted 27 June 2012 - 12:15 AM

Hi friends,


It sounds vey stupid but anyway!
My friend told me that there is difference between the protein and DNA electrophoresis-
For SDS-PAGE -- we take the top edge of bands as the exact weight of protein
For DNA - we take the bottom edge of bands as the exact weight of protein

Please clarify me on this.

#2 leelee

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Posted 27 June 2012 - 07:27 AM

I think it is irrelevant which side of the band you chose to measure from- as long as you are consistent.
So if you use the bottom edge of the band for your molecular weight standards, you use the bottom edge for your unknown samples too :)





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