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help for 3d cell culture bca

collagen BCA assay ALP assay protein 3d cell culture

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#1 melissamonterosso

melissamonterosso

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Posted 26 June 2012 - 01:17 PM

Hi
I'm running an experiment with DPSCs in a 3d environment (rat tail collagen type 1 matrix) including A-MEM, FBS, Pen/Strep, and HEPES.
I want to run a BCA and ALP assay on the cells after a week in culture
Do i have to break down the collagen or will it not affect the results of the assays? (collagen is colored yellowish)
If I do have to break it down, what do i use that will not kill the cells, and allow me to simply isolate the proteins so i can see what is going on?
If i don't have to, can i just use the M-PER to lyse the cells and measure the results from that?
thanks





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