I am working on developing a Biotin Avidin based competition ELISA. However, the competition of the biotynylated with the free protein is not giving a decrease in OD with change in conc. of the non biotinyalted protein. I have tried with both Mab and Pab as coating Ab, however i am not able to find a solution to this. Can somebody educate me on this. If you have some reading material on how to develope this Biotin avidin bseed comp ELISA pl let me know.
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Biotin Avidin based Competition ELISA
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