Ampicillin kills my hands!
Posted 25 June 2012 - 08:14 AM
I am a Lab Tech at UF in Gainesville, FL. I study plant pathogens and regularly use Ampicillin aliquots in media.
My question is: Whats the easiest way to get the Ampicillin to pass through the 0.22um filter? I am using 10g of Amp in 20ml of sterile h20.
Everytime I have to make Amp, my hands hurt for days after because I have to force the soultion through the filter and it gives me a lot of resistance.
I read something about using ethanol to make it more soluble or easier to pass through the filter, but was unable to verfiy this. Any and all help is much appreciated.
Thanks in advance
Posted 25 June 2012 - 08:59 AM
If not: can you filter it twice, e.g. first a 2µm filter and then the 0.22; or use an electrical filtration system.....
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Posted 25 June 2012 - 10:47 AM
Posted 25 June 2012 - 12:55 PM
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Posted 26 June 2012 - 03:56 AM