I would expresse a foreign protein in 293T by transient transfection. I want to know how may the weight rotio range of the foreign protein/total protein be. I failed to find some answers to this question，I wonder if someone would share some experience no matter about what kind of cells. thank you.
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interest protein and total protein ratio range
Started by mawson, Jun 24 2012 06:10 PM
293T protein ratio
2 replies to this topic
#2
bob1
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Posted 25 June 2012 - 01:10 PM
It all depends on how good the transfection is and how strong the promoter is for expressing the protein off the plasmid. I would expect that the ratio would be in the range of 1:1,000,000 or lower - often you can't even see a change in band intensity (of the correct size) on a coomassie or ponceau stained gel/membrane following SDS-PAGE of transfected cells.
#3
mawson
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Posted 25 June 2012 - 05:49 PM
bob1, on 25 June 2012 - 01:10 PM, said:
It all depends on how good the transfection is and how strong the promoter is for expressing the protein off the plasmid. I would expect that the ratio would be in the range of 1:1,000,000 or lower - often you can't even see a change in band intensity (of the correct size) on a coomassie or ponceau stained gel/membrane following SDS-PAGE of transfected cells.
