interest protein and total protein ratio range293T protein ratio
Posted 24 June 2012 - 06:10 PM
Posted 25 June 2012 - 01:10 PM
- mawson likes this
Posted 25 June 2012 - 05:49 PM
thank you for help. I used CMV promotor. As you said, I had tried SDS-PAGE and coomassie stain before, but there's no difference between the experiment and control. I supposed the raotio maybe 1:100, and this suppose with out proof maybe so far from the truth.
It all depends on how good the transfection is and how strong the promoter is for expressing the protein off the plasmid. I would expect that the ratio would be in the range of 1:1,000,000 or lower - often you can't even see a change in band intensity (of the correct size) on a coomassie or ponceau stained gel/membrane following SDS-PAGE of transfected cells.