bob1, on 25 June 2012 - 01:10 PM, said:
It all depends on how good the transfection is and how strong the promoter is for expressing the protein off the plasmid. I would expect that the ratio would be in the range of 1:1,000,000 or lower - often you can't even see a change in band intensity (of the correct size) on a coomassie or ponceau stained gel/membrane following SDS-PAGE of transfected cells.
thank you for help. I used CMV promotor. As you said, I had tried SDS-PAGE and coomassie stain before, but there's no difference between the experiment and control. I supposed the raotio maybe 1:100, and this suppose with out proof maybe so far from the truth.