I have a big problem on my RNA silence experiment. I try to knock down SR-BI. I have reached 70 percent decrease in mRNA level after 72h transfection, but did not see effect on protein level. Then I tried to do transfection twice at 72h interview. First, I transfect in 12 well plate, after 72h, I did transfection again, but scaled to 6 well plate because I got 80-90 percent confluence of cells in 12 well plate. However, I still do not get effect on protein level, and I saw many cells were dead. I do not know what to do next. Is there any problem with my protocol? Could anyone give some suggestions?
Thanks a lot!
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Can not reduce protein level
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