Precipitation during dialysisdialysis issue
Posted 22 June 2012 - 10:33 AM
Recently, I did some dialysis for coupling reaction. The IgG (protein used for coupling dye) is in PBS buffer contain 30% glycerol. When I added NHS-SS-biotin in IgG solution and finished coupling reaction. I did dialysis against PBS to remove exceed biotin reagent. But I found precipitation in the dialysis tube (from spectrum lab). All process was done at 4C.
My hypothesis is glycerol crystalization??? I am not sure if it is my IgG protein..
Anyone have any idea???
I will run western blot tomorrow...
Posted 22 June 2012 - 11:39 PM
Posted 07 July 2012 - 04:09 AM