Hello.
I'm trying to amplify the b-actin gene in Oncorhynchus mykiss in conventional PCR. I designed the primers with bioinformatic programs but nothing is working, I haven't found primers for DNA for this specie, or similar, like salmo salar... Does anyone have work with this procedure? I need more ideas for the primer design
please, help!
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No PCR amplification with b-actin primers
Started by andrea-a, Jun 22 2012 10:23 AM
PCR b-actin primers
1 reply to this topic
#2
ashu2007
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Posted 22 June 2012 - 01:06 PM
It might be a problem in your PCR master mix or any component of that.
The first thing is, did you get anything in your PCR. I used to design my primers using primer design(applied bio system) and it works for me.
The first thing is, did you get anything in your PCR. I used to design my primers using primer design(applied bio system) and it works for me.
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