Hello.
I'm trying to amplify the b-actin gene in Oncorhynchus mykiss in conventional PCR. I designed the primers with bioinformatic programs but nothing is working, I haven't found primers for DNA for this specie, or similar, like salmo salar... Does anyone have work with this procedure? I need more ideas for the primer design
please, help!
0
No PCR amplification with b-actin primers
Started by andrea-a, Jun 22 2012 10:23 AM
PCR b-actin primers
1 reply to this topic
#2
Curlis
-
- Active Members
-
- 25 posts
member
0
Neutral
Neutral
Posted 22 June 2012 - 01:06 PM
It might be a problem in your PCR master mix or any component of that.
The first thing is, did you get anything in your PCR. I used to design my primers using primer design(applied bio system) and it works for me.
The first thing is, did you get anything in your PCR. I used to design my primers using primer design(applied bio system) and it works for me.
Also tagged with one or more of these keywords: PCR, b-actin, primers
 |
Protocols and Techniques Forums →
ChIP and Next Generation Sequencing →
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
qPCR question (no results?)Started by Guest_LQJ_* , 31 May 2013  PCR, qPCR, real-time and 2 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Artificial RNA as normalizer (HK gene)Started by Guest_detriar_* , 26 May 2013 real-time pcr, pcr and 3 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
High fidelity PCR trouble shootingStarted by Guest_uday_* , 23 May 2013 high fidelity, pcr
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Reproducible Non-Specific PCR ProductStarted by Guest_Epigeneticist_* , 20 May 2013 PCR, Sequencing, Cloning
|
|
|
