Hello everybody,
I have been doing 2D gel electrophoresis for my yeast protein to identify its posttranslational modifications with a combination of 7M urea and 2M thiourea in my IPG rehydration buffer (at room temperature), unless I came through carbamylation as an artifact from urea. I am afraid if my protein undergoes carbamylation in vitro. I read that guanidine hydrochloride and/or phosphates can be used to avoid carbamylation in vitro. Does anybody knows and can suggest the composition of buffers containing guanidine hydrochloride and/or phosphates?
I would be very thankful for your help.
Wishes,
Manisha.
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Guanidine Hydrochloride and/or Phosphates Containing IPG rehydration buffer for
Started by manishasaxena18, Jun 22 2012 01:00 AM
2D sample guanidine HCL Phosphate IPG buffer
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Also tagged with one or more of these keywords: 2D sample, guanidine HCL, Phosphate, IPG buffer
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