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Guanidine Hydrochloride and/or Phosphates Containing IPG rehydration buffer for

2D sample guanidine HCL Phosphate IPG buffer

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#1 manishasaxena18

manishasaxena18

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Posted 22 June 2012 - 01:00 AM

Hello everybody,

I have been doing 2D gel electrophoresis for my yeast protein to identify its posttranslational modifications with a combination of 7M urea and 2M thiourea in my IPG rehydration buffer (at room temperature), unless I came through carbamylation as an artifact from urea. I am afraid if my protein undergoes carbamylation in vitro. I read that guanidine hydrochloride and/or phosphates can be used to avoid carbamylation in vitro. Does anybody knows and can suggest the composition of buffers containing guanidine hydrochloride and/or phosphates?

I would be very thankful for your help.

Wishes,
Manisha.





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