2 replies to this topic

[Winnie]

Hi,I would like to know how to calculate RNA for making cdna and what do u mean by diluting the RNA,
I have omniscript RT kit and will be using SYBR Green as well .. this is not for any sample but just learning these techniques..

[bob1]

What do you mean calculate?  You should know the concentration of your RNA and from that you should be able to work out how much you need for the cDNA reaction.  Diluting means making a lower concentration by adding more solution, whether it be water or some buffer.

[Winnie]

Thanks a lot ..will do that..
Cheers