MEDIA REDOX STATUS
Posted 21 June 2012 - 01:37 AM
Background: My stem cell lines behave very differently in two different media and I suspect this has something to do with how my cell's redox homeostasis is being influenced by the media.
Question: is there a simple way of calculating whether a media confers a more reduced or more oxidized environment on the cells? The one media I am using is IMDM:F12 and the other is ADF (advanced DMEM F12), both marketed by Invitrogen.
Many thanks in advance for any suggestions
Posted 21 June 2012 - 01:31 PM
Posted 21 June 2012 - 10:36 PM
Thanks bob1. How about components such as glutathione and ascorbic acid. Wouldn't these components in the media generate cells with a more reduced intracellular milieu?
Posted 24 June 2012 - 02:24 PM