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DNA fragmentation


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#1 majidatf

majidatf

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Posted 20 June 2012 - 11:30 AM

Hi to All

I am trying to assess apoptosis induction by DNA fragmentation (DNA laddering).
I lysed my cells by lysis buffer and purified DNA by Phenol-chloroform- isoamylalcohol. But unfortunately I have'nt detect the ladder until now. I seed the cells in 6 well duplicate for each concentration. and I run 6microgram DNA in agarose gel .
I would be glad and appreciated If anybody introduce a sufficient protocol.
thanks

#2 JYaron

JYaron

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Posted 02 January 2013 - 11:05 PM

Have you tried everything in this protocol?

http://www.immunolog...OOK98/CHAP4.HTM
Jordan Yaron, PhD Candidate
The Biodesign Institute at Arizona State University

www.jordanyaron.com

Ask me about cell biology, microscopy and cell death or find my writing at TheBioBlog.com!




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