Hi to All
I am trying to assess apoptosis induction by DNA fragmentation (DNA laddering).
I lysed my cells by lysis buffer and purified DNA by Phenol-chloroform- isoamylalcohol. But unfortunately I have'nt detect the ladder until now. I seed the cells in 6 well duplicate for each concentration. and I run 6microgram DNA in agarose gel .
I would be glad and appreciated If anybody introduce a sufficient protocol.
thanks
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JYaron
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PhD Candidate, Biological Design
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Posted 02 January 2013 - 11:05 PM
Jordan Yaron, PhD Candidate
The Biodesign Institute at Arizona State University
www.jordanyaron.com
Ask me about cell biology, microscopy, caspase-1-dependent inflammation and cell death or find my writing at TheBioBlog.com!
The Biodesign Institute at Arizona State University
www.jordanyaron.com
Ask me about cell biology, microscopy, caspase-1-dependent inflammation and cell death or find my writing at TheBioBlog.com!
