Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

culturing MDA-MB-134


  • Please log in to reply
1 reply to this topic

#1 kevi_kevi

kevi_kevi

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 20 June 2012 - 02:37 AM

Hello, I've been having trouble with my MDA MB 134 cells. I know that ATCC recommends them to be cultured in L15+20% FCS, but our lab does not have a non-CO2 incubator...I read somewhere (can't find the reference paper now!! which is very annoying) that I could culture them in DMEM/F12 (1:1) with 10% FBS, but they haven't really been growing from when I brought them up from the vial I received from ATCC...

Any help??

#2 Natrium

Natrium

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 20 June 2012 - 09:14 PM

If the problem is not having a non-CO2 incubator, can you just close the cap all the way so it is air-tight? This won't work if you have the perforated caps, but if you have the caps that you unscrew a quarter turn in the incubator, it seems like this should work fine.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.