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Fractionation of Cytoplasmic and Nuclear proteins


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#1 alyjan

alyjan

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Posted 19 June 2012 - 10:49 AM

I need to separate cytoplasmic and nuclear proteins in PBMCs for western blotting. Does anyone have information on a protocol for doing this?

Also, does anyone know approximately how much cytoplasmic protein and nuclear protein can be extracted from 1 million PBMCs?

Thanks




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