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Cell lysis buffer for His-pull down

Pull-down Histidine Protein purificatio Lysis buffer

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#1 Mark_lab

Mark_lab

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Posted 19 June 2012 - 07:54 AM

Hi,

i would like to perform a Histidine-pull down assay but i have a problem with the extraction buffer.

this buffer should not disrupt protein-protein interactions for that i have to use non-ionic detergents. Also, it should not have EDTA or similar compounds because disrupts the binding to magnetic beads.

I have tried to find a commercial buffer with this characteristics but i have not found it.

someone knows about a commercial buffer which this characteristics? or even a handmade buffer which works for this assay?

Thks





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