I am new in qPCR and have recently got this kit that requires instrument specific optical qPCR plates. The instrument we have in the lab is the MJ research Opticon 2 machine. As far as I am aware it only takes 96 well plates. Checked online on various websites: fisher, sigma. However they all have qPCR plates that range from different colours to different types like skirted or unskirted. Can anyone advice me as to which kind of plate in terms of colour and skirting I need for the qPCR?
Hope to hear from anyone soon.
0
optical qPCR plates
Started by CMIRC, Jun 19 2012 07:32 AM
pcr qpcr rtpcr molecular biology genetics
1 reply to this topic
#2
Trof
-
- Global Moderators
-
- 946 posts
Brain on a stick
66
Excellent
Excellent
Posted 21 June 2012 - 05:52 AM
Manufacturers should specify the recommended plate type for each cycler. Like here from AB gene (check Compatibility table).
Color usualy depends on the material. White/opaque materials are said to have better optical characteristics but it's difficult to see what you pipett to it in contrast with transparent plates. The color of opaque plates itself play no role, it's just a brand thing IMHO.
Color usualy depends on the material. White/opaque materials are said to have better optical characteristics but it's difficult to see what you pipett to it in contrast with transparent plates. The color of opaque plates itself play no role, it's just a brand thing IMHO.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
I never trust anything that can't be doubted.
Also tagged with one or more of these keywords: pcr, qpcr, rtpcr, molecular biology, genetics
 |
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
High fidelity PCR trouble shootingStarted by Guest_uday_* , 23 May 2013  high fidelity, pcr
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Reproducible Non-Specific PCR ProductStarted by Guest_Epigeneticist_* , 20 May 2013 PCR, Sequencing, Cloning
|
|
|
|
|
|
Protocols and Techniques Forums →
Tissue and Cell Culture →
Housekeeper for HEK cells?Started by Guest_Stephp_* , 17 May 2013 HEK cells, house keeper, PCR and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Cloning large fragmentsStarted by Guest_bknm_* , 15 May 2013 Cloning, PCR, large inserts
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Can't get PCR with large overhang primers to workStarted by Guest_EtOH_* , 10 May 2013 PCR, overhang, low gc
|
|
|
