2 replies to this topic

[Brauseziege]

Hi all,
I was just scouring the internet for information about a vitality test, stumbled across this forum and was wondering if you would be able to help:
In our research group we work with the murine retina and at the moment I want to establish a new assasy.
Therefore I cut retinal slices (200µm) with a vibratom and now want to check the vitality of the cells in the slices.
Has anyone an idea what reagent I could use? Is trypan blue a solution or is it only for dissociated cells or cell culture?
Thank you and looking forward to your answers,
Anna

[bob1]

Trypan blue could work, you could possibly also use neutral red which only stains viable cells.

[Brauseziege]

Thanks bob,we have trypan blue here in our lab, I think I´ll just try it today and when it doesn´t work, will look for something else!