Vitality test for retinal slices
Posted 19 June 2012 - 07:27 AM
I was just scouring the internet for information about a vitality test, stumbled across this forum and was wondering if you would be able to help:
In our research group we work with the murine retina and at the moment I want to establish a new assasy.
Therefore I cut retinal slices (200µm) with a vibratom and now want to check the vitality of the cells in the slices.
Has anyone an idea what reagent I could use? Is trypan blue a solution or is it only for dissociated cells or cell culture?
Thank you and looking forward to your answers,
Posted 20 June 2012 - 05:58 PM
Posted 21 June 2012 - 12:16 AM