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target mRNA/protein levels increase with siRNA

siRNA

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#1 FACSaddicted

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Posted 15 June 2012 - 02:41 AM

Dear all

I am having a problem with my siRNA experiments. I am using primary DC cells that express GFP and I am trying to know down GFP expression. My readout if flow cytometry and q-PCR. In some of my experiments I have an increase of GFP when adding siRNA GFP when compared to siRNA control. Do you have any ideas why I have such a contradictory results?

Thank you so much for your help.

#2 Jon Moulton

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Posted 15 June 2012 - 05:52 AM

I've heard of small dsRNA increasing expression. Here's a paper that might start you into the literature of this topic: http://www.nature.co...h0208-122a.html
Jon D. Moulton
Gene Tools, LLC
www.gene-tools.com

#3 FACSaddicted

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Posted 15 June 2012 - 07:53 AM

Thank you for your answer. I will take a look at the paper.





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