Using unopened restriction enzyme "expired" in 2010/11
Posted 14 June 2012 - 08:07 PM
Would a restriction enzyme stored untouched at -20C be viable even after it's use-by-date?
Anyone who has used it before and seen that it stills cut perfectly?
Posted 14 June 2012 - 09:29 PM
You could just give it a go and see?
Posted 15 June 2012 - 01:56 AM
As long as the enzymes have been stored in consistent standard conditions they're almost always fine.
I'd suggest running a little test of them - if they work, they work. If in doubt throw them out.
If, however, you work in a regulated (that is, accredited) laboratory you'll have to look at if you can use these enzymes according to your system.
Speak to your supervisor.
Posted 20 June 2012 - 04:47 AM