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Need help for Intrinsic tryptophan fluoroscence

Trp fluoroscence

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#1 jalapati

jalapati

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Posted 14 June 2012 - 07:26 AM

Hi,
I am Jyothirmai. Graduate student majoring Biochemistry.
Can anyone please help me out to understand Intrinsic tryptophan fluoroscene method?
Why we use this method and what are the applications of this method?
I am working on the protein glycoprotein 96 (molecular chaperone, hsp). Can we use this method to check conformational changes that are occuring in our protein when subjected to heat shock?
Thank you.




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