Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Genewiz Sequencing Problem

no priming

  • Please log in to reply
No replies to this topic

#1 HOYAJM

HOYAJM

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 159 posts
12
Good

Posted 14 June 2012 - 06:25 AM

Hi, I have submitted a plasmid to Genewiz for sequencing on 3 occasions. All three times the plasmid was not sequenced due to "no priming". The only thing they tell me is that it can be a concentration problem or contamination. The plasmid I have sent has come from miniprep's and maxiprep's and looks identical to the original plasmid when run on a gel. I have used universal primers from Genewiz (T7 and Neomycin) and neither worked due to no priming (the plasmid has these sites). Has anyone had a similar problem and how did you overcome it? Any advice would be greatly appreciated.

Side note. I have linearized the plasmid in separate reactions just fine. So I doubt there are inhibitors in the preps.

Thanks




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.