I have isolated RNA and used 2 ug of RNA to perform RT-PCR and i have a primer for my target gene for qpcr. I want to ask if i can use the same primer to do conventional PCR (agarose gel) or semiquantitative PCR?
Do i need to quantify my cDNA and load the same amount of template for PCR? or just take equal volume from my RT-PCR product? How can i make sure that my semiquantitative PCR run can be comparable from each samples?
i hope you can help me with these basics. thank you...
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