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Denaturation of DNA blotted


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#1 Anap

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Posted 13 June 2012 - 03:35 PM

Hi everyone.

After I finished and crosslinked my blot I realized that I completly forgot the depurination and desnaturation before blotting.

There is any way to use that membrane? It is for a sourthern blot using DIG

#2 bob1

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Posted 14 June 2012 - 01:47 PM

Probably not - you might as well just repeat it anyway, you will have to do this no matter what result you get as you can't be sure whether the result is due to the technical error or not.




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