1 reply to this topic

[jmafs]

Hi all,

I'm new to process of siRNA transfection and I was wondering: Will one siRNA sequence (previously validated in the lab) be good enough to transfect multiple other cell lines from the same organism?  I understand that the actual process of transfection will be different for each cell line, but I am curious as to whether I need to also worry about the sequence itself.

Thanks!

[Anexum]

jmafs, on 13 June 2012 - 09:41 AM, said:

Hi all,

I'm new to process of siRNA transfection and I was wondering: Will one siRNA sequence (previously validated in the lab) be good enough to transfect multiple other cell lines from the same organism?  I understand that the actual process of transfection will be different for each cell line, but I am curious as to whether I need to also worry about the sequence itself.

Thanks!

Sequence shouldnt be a problem for transient or stable transfections tbh, you may need to change the vector but apart from that the sequence should not change.
Depending on the gene its knockdown could kill the cell,though tbh, that is a rare occurance and is usually only if its a cell signalling protien key to anti-apoptotic signalling