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MTT assay in DRG culture

cytotoxcity Neuroscience Cell culture

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#1 Rangus

Rangus

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Posted 13 June 2012 - 03:55 AM

Can someone please help me!

I am trying to do a MTT assay in my DRG cultures, but so far it isn't working. I get some purple crystals forming after MTT incubation, but after adding my solvent i don't get any change in colour. I was wondering if it if might be due to my low plating density.

Does anyone have a protocol for doing the MTT assay in DRG cultures?

Thank you!





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