Hello everyone, i am new in molecular biology and i would like you to help.
i actually want to assess the effect of some chemicals on a cluster of 4 genes (6kb) although two of them seem to be more necessary than others, for the moment i do not know if the best way is to assess the mRNA production or proteins.
second do i need to take out these genes and use a vector for the experiment ?
i have got sequences of my 4 genes from NCBI and i assume ORFs are included and my genes can be expressed.
regards
ott
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5 replies to this topic
#1
Posted 13 June 2012 - 03:46 AM
#2
Posted 13 June 2012 - 01:16 PM
a) it depends on what sort of result you want - ideally you should do both.
b)Maybe, is there endogenous expression?
b)Maybe, is there endogenous expression?
#4
Posted 21 June 2012 - 08:52 AM
thanks guys i will try both
but i still have a question do we need to make constructs to assess the level of expression?
but i still have a question do we need to make constructs to assess the level of expression?
#5
Posted 21 June 2012 - 01:17 PM
For endognous expression, you would look for the expression from the cell only, so no transfected constructs.
#6
Posted 17 July 2012 - 06:08 AM
Thank you!
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