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The Differences Between CHO DG44 and CHO dhfr-

DG44 dhfr- CHO difference transfection

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#1 chan8



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Posted 12 June 2012 - 08:50 AM

I am looking to purchase DG44 cells because these are the cells that I see appear in the literature most often for transient transfection and recombinant protein production for commercial uses. I know that DG44 cells are dhfr- mutants, but the only vendor I see refer to their cells as DG44 is at Invitrogen. All other cell line vendors just refer to them as CHO dhfr-. Is there an actual difference do you think between cells designated DG44 and those designated CHO dhfr-? Thanks!

#2 bob1


    Thelymitra pulchella

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Posted 12 June 2012 - 02:37 PM

It seems that they are probably the same cell line - Urlaub and Chasin seem to be the names mentioned with them, and they were at least collaborators on the paper describing a DHFR- CHO cell line.

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