Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

DNA Index?

dna index telomere FISH RTL Propidium Iodide

  • Please log in to reply
No replies to this topic

#1 DaviQd



  • Members
  • Pip
  • 1 posts

Posted 11 June 2012 - 06:01 PM


I'm attempting to determine relative telomere length (RTL) using Dako's Telomere PNA Kit <http://www.dako.com/...d_products.htm> and am having trouble understanding what the term DNA index means. The formula for RTL on Page 9 of the protocol <http://www.dako.com/...ctid=114791002> requires the DNA index of my control & sample cells. The ratio of aneuploid/euploid appears to be what "DNA index" refers to. Is this correct?

Earlier in the protocol I'm told to gate for G0/1 cells [using a FL-A vs FL-W plot]. If I've already gated for all the 2C cells, why does the formula require DNA index? This seems redundant to me, but I'm probably just misunderstanding something.

Thanks everyone!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.