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DNA Index?

dna index telomere FISH RTL Propidium Iodide

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#1 DaviQd

DaviQd

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Posted 11 June 2012 - 06:01 PM

Greetings,

I'm attempting to determine relative telomere length (RTL) using Dako's Telomere PNA Kit <http://www.dako.com/...d_products.htm> and am having trouble understanding what the term DNA index means. The formula for RTL on Page 9 of the protocol <http://www.dako.com/...ctid=114791002> requires the DNA index of my control & sample cells. The ratio of aneuploid/euploid appears to be what "DNA index" refers to. Is this correct?

Earlier in the protocol I'm told to gate for G0/1 cells [using a FL-A vs FL-W plot]. If I've already gated for all the 2C cells, why does the formula require DNA index? This seems redundant to me, but I'm probably just misunderstanding something.

Thanks everyone!





Also tagged with one or more of these keywords: dna index, telomere, FISH, RTL, Propidium Iodide

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