No replies to this topic

[DaviQd]

Greetings,

I'm attempting to determine relative telomere length (RTL) using Dako's Telomere PNA Kit <http://www.dako.com/...d_products.htm> and am having trouble understanding what the term DNA index means.  The formula for RTL on Page 9 of the protocol <http://www.dako.com/...ctid=114791002> requires the DNA index of my control & sample cells.  The ratio of aneuploid/euploid appears to be what "DNA index" refers to.  Is this correct?

Earlier in the protocol I'm told to gate for G0/1 cells [using a FL-A vs FL-W plot].  If I've already gated for all the 2C cells, why does the formula require DNA index?  This seems redundant to me, but I'm probably just misunderstanding something.

Thanks everyone!