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Which proteasome activity assay is best?

proteasome activity mammalian cells

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#1 elcain

elcain

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Posted 11 June 2012 - 04:25 PM

Hi!

I want to find out if expression of my GOI affects proteasome activity in a mammalian cell line. I see a variety of kits and protocols for measuring proteasome activity. I was wondering if anyone working with the proteasome could provide a recommendation for a protocol or kit for measuring proteasome activity. I was thinking of trying the Cell-Based Proteasome-Glo kit from promega for starters, but am also thinking of doing a native gel and using a fluorgenic substrate. Any thoughts would be appreciated!





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