Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

1 votes

Endogenous protease cleavage in E. coli

Started by Esrop, Jun 11 2012 02:22 AM

protease cleavage site coli endogenous

Please log in to reply

No replies to this topic

#1 Esrop

member

Members
1 posts

0
Neutral

Posted 11 June 2012 - 02:22 AM

Hallo all,

I am designing a protein linker which has to be cleaved at a (non sterically hindered) position between two proteins in E. coli.
Does anyone know any specific cleavage sites for endogenous proteases in E. coli?

Thanks in advance!

Back to Molecular Biology · Next Unread Topic →

Also tagged with one or more of these keywords: protease, cleavage site, coli, endogenous

Protocols and Techniques Forums → Immunology → ELISA and Immunoassay → ELISA positive result from Phosphatase/Protease inhibitor? Started by Rnotk, 15 Feb 2018 ELISA, Protease, false positive	3 replies 1,094 views	bob1 16 Feb 2018
Student Forums (for study, homework and non technical questions) → -Molecular Biology- → Concentration of protease from bacillus sp. in a liquid form? Started by brizzles, 10 Jul 2015 protease, bacillus sp, liquid and 1 more...	1 reply 2,008 views	mdfenko 13 Jul 2015
Protocols and Techniques Forums → SDS-PAGE and Western Blotting → Western blot detects exogenous protein but not endogenous Started by 5280, 12 Apr 2015 Western blot, Endogenous and 1 more...	2 replies 1,082 views	5280 12 Apr 2015
Protocols and Techniques Forums → Protein Expression and Purification → regarding protein purification using 8m urea and sds page Started by atulp, 19 Apr 2014 protease	2 replies 1,416 views	labtastic 06 May 2014
Protocols and Techniques Forums → Molecular Cloning → difficulty to clone and/or express proteases. Toxicity? Started by monicalvi, 19 Apr 2013 protease, dh5 alpha, induction and 1 more...	0 replies 1,337 views	monicalvi 19 Apr 2013