hi
in your experiences what are the common staining artifacts observed while staining proteins (silver,commassie etc..) in polyacrylamide gel ??
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#2
Inmost sun
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Posted 11 June 2012 - 11:38 PM
I think traces of lipids can cause staining background...
#3
mdfenko
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Posted 14 June 2012 - 11:56 AM
nucleic acids stain with silver (we used to use trna to coprecipitate with low concentration proteins, stopped using it when we started to stain with silver and got bright orange blobs)
an artifact, presumably attributed to keratins from dust, shows up in the 50-70 kDa range.
an artifact, presumably attributed to keratins from dust, shows up in the 50-70 kDa range.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
