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Vertical Vs horizontal electrophoresis


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3 replies to this topic

#1 rick112

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Posted 10 June 2012 - 11:29 AM

HI

on comparison of vertical and horizontal electrophoresis what are ones advantages and disadvantages ???

#2 bob1

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Posted 10 June 2012 - 03:33 PM

For what samples?

#3 rick112

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Posted 11 June 2012 - 05:16 AM

in general, what is the advantage of one over the other ???

#4 bob1

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Posted 11 June 2012 - 01:55 PM

It depends on what you are trying to do - (assuming you mean acrylamide gels here too) vertical generally = thin gels, good resolution, but smaller sample volumes. horizontally= thick gel, poorer resolution but much bigger samples, easier to access for cutting out bands/band elution.




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