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why am I getting high km value for a enzyme


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#1 jaya2020

jaya2020

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Posted 10 June 2012 - 10:40 AM

Hi,
Iam using xenopus oocyte to express my protein. Iam getting a Km value twice that has been reported for the same protein in the same expression system. The experiment has been repeated over and again, but I get the same value. I really dont know what to do now. Please help. Thanks in advance.

regards,
jaya




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