Dear friends!
I need your advice regarding the engineering of di-sulfide bond.
The task is following: I have a complex of 2 proteins. I would like to increase it's stability bu introducing an artificial di-sulfide bond between two proteins. I know approximately the residues of both proteins that should be located in proximity to each other. I know how to make site-directed mutagenesis. Could you recommentd any reading on this topic? I.e. protocols, research articles etc etc. or share your experience? What should be the expresssion conditions in this case?
Ingineered di-sulfide bond - how-to?
Started by Goggi, Jun 09 2012 05:11 AM
di-sulfide bond protocol how-to
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