Dear friends!
I need your advice regarding the engineering of di-sulfide bond.
The task is following: I have a complex of 2 proteins. I would like to increase it's stability bu introducing an artificial di-sulfide bond between two proteins. I know approximately the residues of both proteins that should be located in proximity to each other. I know how to make site-directed mutagenesis. Could you recommentd any reading on this topic? I.e. protocols, research articles etc etc. or share your experience? What should be the expresssion conditions in this case?
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Ingineered di-sulfide bond - how-to?
Started by Goggi, Jun 09 2012 05:11 AM
di-sulfide bond protocol how-to
1 reply to this topic
Also tagged with one or more of these keywords: di-sulfide bond, protocol, how-to
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