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Ingineered di-sulfide bond - how-to?

di-sulfide bond protocol how-to

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#1 Goggi

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Posted 09 June 2012 - 05:11 AM

Dear friends!

I need your advice regarding the engineering of di-sulfide bond.
The task is following: I have a complex of 2 proteins. I would like to increase it's stability bu introducing an artificial di-sulfide bond between two proteins. I know approximately the residues of both proteins that should be located in proximity to each other. I know how to make site-directed mutagenesis. Could you recommentd any reading on this topic? I.e. protocols, research articles etc etc. or share your experience? What should be the expresssion conditions in this case?

#2 PhDinAcronyms

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Posted 16 July 2012 - 08:59 PM

Here are some papers:
http://www.ncbi.nlm....cles/PMC125700/
http://onlinelibrary...03.00348.x/full





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