I would like to make some siRNAs based on published shRNA sequences. The paper indicates that "Two shRNA expression cassettes were generated that targeted P56 mRNA at nt 12–41 (shRNA1) and nt 1443–1472 (shRNA2)"
Is it obvious which 19-21bp sequence would make effective siRNA based on these 30bp sequences?
siRNA based on published shRNAsirna design
No replies to this topic