Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

DAPI and Alexa Fluor 488

DAPI 488 green Hoechst immunocytochemistry

  • Please log in to reply
1 reply to this topic

#1 jeee



  • Active Members
  • Pip
  • 10 posts

Posted 06 June 2012 - 11:05 AM

Quick question. Last time I did immunocytochemistry I used DAPI, Alexa Fluor 488 and 594. The DAPI and 594 came out fine, however 488 was not visible. Since, I've heard that DAPI will mask 488 and that Hoechst will not. Can anyone confirm or elaborate? Thanks

P.S. I am assuming that the receptor I was looking for with the 488 is present.

#2 gyma



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 146 posts

Posted 01 August 2012 - 11:42 PM

in my experience, DAPI and alexa 488 always work well. one blue and one green, always clear. there must be other reasons for your observation.

Also tagged with one or more of these keywords: DAPI, 488, green, Hoechst, immunocytochemistry

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.