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To reduce or no blood leakage into lungs during inflation

Lung inflation

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#1 koksvedan

koksvedan

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Posted 06 June 2012 - 07:38 AM

Hello,

I am working on inflation of mouse lungs with 10% neutral buffered formalin. I am cutting the abdominal arota for the blood to leakout before opening the diaphragm and inflating the lungs.

But when i did collagen staining on the inflated lung tissue which is paraffin embedded i find blood in the lung parenchyma and red dots in the parenchyma.

I would be of great help to know how can i reduce the blood getting into the lung parenchyma and a better way to inflate lungs.

Thanks in advance




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